The virtual rail train usually increases the passenger carrying capacity by equipping and articulating the carbodies, and thus has the long vehicle body with multiple axles and the heavy axle load. During the motion, the train has complex overall and local vibration characteristics under the influence of various factors, which can worsen the ride comfort of the train. In this research, a vertical half-vehicle model of the n-unit train was established in Newton's second law with consideration of the human-seat spatial distribution of the train and the random irregularity excitations of the road, in order to comprehensively evaluate the ride comfort of the train. As an example, the ride comfort analysis of a three-unit train was carried out with the train model established. For its purpose, the vertical and pitch acceleration responses of the system were firstly analyzed in both time and frequency domains, then the effects of the factors, including the road surface grades, the operating speed, the number of carriages, the suspension parameters and the articulation parameters between articulated carbodies, were then examined in terms of the weighted acceleration root mean square index aw of the seats. The obtained results show that the middle carbody has the better ride comfort while the head and tail carbodies have the similar comfort levels. The ride comfort of the train can be improved by enhancing the road grade, reducing the train speed and increasing the number of carbodies. Moreover, it indicates that the ride comfort of the train is highly sensitive to the suspension parameters, and the large articulation damping between the carbodies has a certain positive effect on the train ride comfort.