The vibration characteristic of the bi-material cantilever beam with graded interface layer was studied by Timoshenko beam corrective theory. The neutral axis site of the bi-material cantilever beam with graded interface layer was determined by the static equilibrium equations and the vibration equations of bi-material cantilever beam with graded interface layer were also established by Timoshenko beam corrective theory, the expression for natural frequency of it and the analytical solution for forced vibration of it under the action of harmonic load were obtained. The effect of neutral axis site to vibration characteristic of bi-material cantilever beam with graded interface layer was discussed. Analysis of examples indicates that the height of graded interface layer had more greatly influence on vibration characteristic of bi-material cantilever beam with graded interface layer.